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产品目录

Bso31I内切酶图片
产品货号:
GS2197
中文名称:
Bso31I内切酶
英文名称:
Bso31I
产品规格:
500U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sitesGGTCTC(N)1↑
来源Bacillus stearothermophilus 31
Assayed onT7 DNA
Optimal bufferO(50mMTris-HCl(pH 7.6 at 25℃);10 mM MgCl2;100 mM NaCl;1 mM DTT.)+ BSA
Optimal temperature55℃
Storage conditions10 mM Tris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100μg/ml BSA;50%glycerol;Store at -20℃.
LigationsAfter 5-fold overdigestion with enzyme more than 90%of DNA fragments can be ligated.Of these 80%can be recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a.of enzyme for 16hoursat 55℃.
Methlation effectNot blocked by methylation GGTCTmC
Thermal inactivationenzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible EndsBsaI,BspTNI,Eco31I

相关搜索:Bso31I内切酶Bso31I限制性内切酶
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